Variant

Genome Chromosome Position VCF ID Ref Alt mRNA Change mRNA Info
GRCh37 chr6 31545258 . G A CCDS4702.1:NM_000594.3:c.646Gac>Aac_NP_000585.2:p.216D>N Homo sapiens tumor necrosis factor (TNF), mRNA.
pdb_id: 
label_asym_id: 
label_seq_id: 
label_comp_id: 
auth_asym_id: 
auth_seq_id: 
alphafold_id: 
label_asym_id: 
label_seq_id: 
label_comp_id: 
auth_asym_id: 
auth_seq_id: 

PDB

Entity Residue Monomer BioUnit Ligand View
PDB Entity Uniprot Identity Evalue ExpMethod Date Name Label Auth ASA rASA SS φ ψ ASA rASA ΔASA Interaction Name Distance Atom(p) Atom(l)
6op0 1 P01375 99.37 6e-115 X-RAY
 2019-12-25 ASP A:141 A:140 106.0 0.707 G -64.9 -13.9 106.0 0.707 0.0 HOH
 4.136
 O
 O
ASP B:141 B:140 101.0 0.673 G -59.3 -16.9 101.0 0.673 0.0 HOH
 7.343
 O
 O
ASP C:141 C:140 99.0 0.66 T -74.5 -22.6 99.0 0.66 0.0
7kp9 1 P01375 99.37 6e-115 X-RAY
 2021-01-13 ASP A:141 A:140 100.0 0.667 G -64.8 -17.7 100.0 0.667 0.0 HOH
 4.901
 OD2
 O
ASP B:141 B:140 102.0 0.68 G -64.0 -24.5 102.0 0.68 0.0 HOH
 4.087
 O
 O
ASP C:141 C:140 100.0 0.667 G -56.1 -10.8 100.0 0.667 0.0 HOH
 9.068
 N
 O
7kpa 1 P01375 99.37 6e-115 X-RAY
 2021-01-13 ASP A:141 A:140 101.0 0.673 T -66.9 -20.3 101.0 0.673 0.0 HOH
 5.235
 OD2
 O
ASP B:141 B:140 105.0 0.7 G -62.4 -27.0 105.0 0.7 0.0 HOH
 3.694
 O
 O
ASP C:141 C:140 101.0 0.673 G -69.3 -12.1 101.0 0.673 0.0
7kpb 1 P01375 99.37 6e-115 X-RAY
 2021-01-13 ASP A:141 A:140 105.0 0.7 G -63.4 -25.2 101.0 0.673 0.027 G:None:0.027
 GOL
 7.841
 N
 O2
ASP B:141 B:140 98.0 0.653 G -75.1 6.3 98.0 0.653 0.0 GOL
GOL
 7.518
4.565
 N
OD1
 C3
O3
ASP C:141 C:140 105.0 0.7 G -68.5 -11.8 105.0 0.7 0.0
5mu8 1 P01375 100.0 9e-115 X-RAY
 2017-03-29 ASP A:142 A:140 96.0 0.64 G -50.7 -35.3 96.0 0.64 0.0
ASP B:142 B:140 104.0 0.693 G -58.9 -32.6 104.0 0.693 0.0
ASP C:142 C:140 103.0 0.687 G -56.8 -27.2 NA NA NA
ASP D:142 D:140 96.0 0.64 T -62.5 -19.1 NA NA NA
ASP E:142 F:140 98.0 0.653 G -53.3 -27.8 NA NA NA
ASP F:142 G:140 101.0 0.673 G -52.5 -30.2 NA NA NA
ASP G:142 H:140 93.0 0.62 G -53.2 -24.4 NA NA NA
ASP H:142 I:140 97.0 0.647 G -55.9 -31.4 NA NA NA
ASP I:142 J:140 97.0 0.647 T -34.6 -39.5 NA NA NA
ASP J:142 K:140 104.0 0.693 T -54.0 -22.7 NA NA NA
ASP K:142 L:140 91.0 0.607 G -47.5 -33.8 NA NA NA
ASP L:142 M:140 106.0 0.707 G -55.9 -23.3 NA NA NA
ASP M:142 N:140 102.0 0.68 G -77.7 8.1 NA NA NA
ASP N:142 O:140 97.0 0.647 G -64.4 -11.8 NA NA NA
ASP O:142 P:140 91.0 0.607 G -44.0 -26.6 NA NA NA
ASP P:142 Q:140 100.0 0.667 G -63.8 -6.7 NA NA NA
ASP Q:142 R:140 98.0 0.653 G -48.6 -31.1 NA NA NA
ASP R:142 S:140 102.0 0.68 G -54.8 -23.3 NA NA NA
ASP S:142 T:140 102.0 0.68 T -70.6 -24.0 NA NA NA
ASP T:142 U:140 100.0 0.667 G -67.8 -25.7 NA NA NA
ASP U:142 V:140 98.0 0.653 G -58.1 -34.3 NA NA NA
ASP V:142 W:140 106.0 0.707 G -63.6 -13.2 NA NA NA
ASP W:142 X:140 107.0 0.713 G -62.7 -25.9 NA NA NA
ASP X:142 Y:140 107.0 0.713 G -40.7 -27.3 NA NA NA
ASP Y:142 Z:140 94.0 0.627 G -53.4 -29.5 NA NA NA
ASP Z:142 a:140 103.0 0.687 G -56.7 -34.4 NA NA NA
ASP AA:142 b:140 91.0 0.607 G -46.4 -35.7 NA NA NA
ASP BA:142 c:140 95.0 0.633 G -63.6 -0.4 NA NA NA
ASP CA:142 d:140 100.0 0.667 G -71.8 3.1 NA NA NA
ASP DA:142 e:140 101.0 0.673 G -71.4 9.1 NA NA NA
ASP EA:142 f:140 107.0 0.713 G -52.4 -43.3 NA NA NA
ASP FA:142 g:140 97.0 0.647 G -47.7 -33.8 NA NA NA
ASP GA:142 h:140 97.0 0.647 G -52.1 -30.7 NA NA NA
ASP HA:142 i:140 97.0 0.647 G -50.2 -33.5 NA NA NA
ASP IA:142 j:140 98.0 0.653 G -44.2 -39.5 NA NA NA
ASP JA:142 k:140 100.0 0.667 G -48.9 -19.4 NA NA NA
ASP KA:142 l:140 105.0 0.7 G -64.7 -7.2 NA NA NA
ASP LA:142 m:140 102.0 0.68 G -52.0 -31.6 NA NA NA
ASP MA:142 n:140 101.0 0.673 G -37.2 -42.0 NA NA NA
ASP NA:142 o:140 99.0 0.66 T -67.2 -40.9 NA NA NA
ASP OA:142 p:140 101.0 0.673 G -55.9 -29.0 NA NA NA
ASP PA:142 q:140 106.0 0.707 G -49.2 -28.1 NA NA NA
ASP QA:142 r:140 100.0 0.667 G -60.8 -31.2 NA NA NA
ASP RA:142 s:140 94.0 0.627 G -53.1 -31.0 NA NA NA
ASP SA:142 t:140 102.0 0.68 G -65.3 -16.2 NA NA NA
ASP TA:142 u:140 100.0 0.667 G -48.0 -27.0 NA NA NA
ASP UA:142 v:140 94.0 0.627 G -53.9 -32.9 NA NA NA
ASP VA:142 w:140 101.0 0.673 G -69.2 -19.8 NA NA NA
6x81 1 P01375 100.0 9e-115 X-RAY
 2021-01-13 ASP A:141 A:140 105.0 0.7 T -71.2 -22.6 105.0 0.7 0.0 HOH
 2.846
 OD1
 O
ASP B:141 B:140 96.0 0.64 T -71.8 -24.8 96.0 0.64 0.0 HOH
 6.363
 N
 O
ASP C:141 C:140 88.0 0.587 T -73.3 -18.9 88.0 0.587 0.0 HOH
 2.820
 OD2
 O
ASP D:141 D:140 88.0 0.587 G -56.2 -33.1 NA NA NA
ASP E:141 E:140 99.0 0.66 G -77.1 -15.6 NA NA NA
ASP F:141 F:140 93.0 0.62 T -76.5 -18.9 NA NA NA
6x82 1 P01375 100.0 9e-115 X-RAY
 2021-01-13 ASP A:141 A:140 100.0 0.667 G -67.6 -20.1 100.0 0.667 0.0 HOH
 3.077
 OD2
 O
ASP B:141 B:140 97.0 0.647 G -65.8 -22.2 97.0 0.647 0.0 HOH
 4.241
 O
 O
ASP C:141 C:140 99.0 0.66 T -76.8 -15.5 99.0 0.66 0.0 HOH
 3.248
 OD2
 O
ASP D:141 D:140 98.0 0.653 G -70.3 -17.5 NA NA NA
ASP E:141 E:140 100.0 0.667 G -70.8 -15.7 NA NA NA
ASP F:141 F:140 98.0 0.653 T -71.9 -19.8 NA NA NA
6x83 1 P01375 100.0 9e-115 X-RAY
 2021-01-13 ASP A:141 A:140 102.0 0.68 G -72.6 -24.2 102.0 0.68 0.0 HOH
 3.938
 O
 O
ASP B:141 B:140 107.0 0.713 G -72.1 -22.5 107.0 0.713 0.0 HOH
 8.593
 OD1
 O
ASP C:141 C:140 106.0 0.707 T -73.0 -22.1 106.0 0.707 0.0 HOH
 4.440
 O
 O
ASP D:141 D:140 104.0 0.693 T -72.7 -23.0 NA NA NA
ASP E:141 E:140 103.0 0.687 T -73.6 -21.5 NA NA NA
ASP F:141 F:140 105.0 0.7 G -73.5 -23.2 NA NA NA
6x85 1 P01375 100.0 9e-115 X-RAY
 2021-01-13 ASP A:141 A:140 91.0 0.607 G -67.2 -25.0 91.0 0.607 0.0
ASP B:141 B:140 100.0 0.667 G -66.1 -24.6 100.0 0.667 0.0
ASP C:141 C:140 99.0 0.66 G -69.4 -21.3 99.0 0.66 0.0
ASP D:141 D:140 90.0 0.6 G -69.3 -22.7 NA NA NA
ASP E:141 E:140 95.0 0.633 G -65.7 -21.7 NA NA NA
ASP F:141 F:140 97.0 0.647 G -65.5 -25.9 NA NA NA
6x86 1 P01375 100.0 9e-115 X-RAY
 2021-01-13 ASP A:141 A:140 99.0 0.66 G -69.8 -28.0 99.0 0.66 0.0 HOH
 4.053
 O
 O
ASP B:141 B:140 96.0 0.64 G -68.4 -27.4 96.0 0.64 0.0
ASP C:141 C:140 98.0 0.653 G -68.1 -27.0 98.0 0.653 0.0
ASP D:141 D:140 93.0 0.62 G -69.6 -29.7 NA NA NA
ASP E:141 E:140 99.0 0.66 G -67.4 -27.5 NA NA NA
ASP F:141 F:140 100.0 0.667 G -67.0 -28.7 NA NA NA
5yoy 1 P01375 100.0 1e-114 X-RAY
 2018-05-09 ASP A:148 A:140 100.0 0.667 G -67.0 -21.0 8.0 0.053 0.614 I:None:0.427
F:None:0.353
 HOH
 4.273
 OD2
 O
ASP B:148 B:140 99.0 0.66 G -66.6 -20.0 9.0 0.06 0.6 D:None:0.34
G:None:0.413
 HOH
 7.089
 N
 O
ASP C:148 C:140 101.0 0.673 G -67.6 -21.0 6.0 0.04 0.633 H:None:0.427
E:None:0.367
 HOH
 6.836
 N
 O
ASP J:148 J:140 99.0 0.66 G -66.3 -21.9 NA NA NA
ASP K:148 K:140 103.0 0.687 G -66.9 -21.3 NA NA NA
ASP L:148 L:140 98.0 0.653 G -66.1 -21.4 NA NA NA
7jra 1 P01375 100.0 1e-114 X-RAY
 2020-12-09 ASP A:143 A:216 102.0 0.68 G -68.9 -19.7 102.0 0.68 0.0 HOH
 4.904
 OD2
 O
ASP B:143 B:216 105.0 0.7 G -69.3 -19.3 105.0 0.7 0.0 HOH
 3.043
 OD2
 O
ASP C:143 C:216 102.0 0.68 G -69.6 -19.2 102.0 0.68 0.0 HOH
 8.559
 CA
 O
4g3y 3 P01375 100.0 1e-114 X-RAY
 2013-03-27 ASP C:140 C:140 85.0 0.567 G -72.4 -9.8 13.0 0.087 0.48 B:None:0.34
A:None:0.26
 HOH
 7.135
 OD1
 O
4twt 1 P01375 100.0 1e-114 X-RAY
 2015-02-04 ASP A:140 A:140 98.0 0.653 G -64.5 -23.0 98.0 0.653 0.0 HOH
 7.811
 O
 O
ASP B:140 B:140 101.0 0.673 G -66.0 -21.9 101.0 0.673 0.0 GOL
HOH
 7.208
9.135
 N
O
 O3
O
ASP E:140 C:140 97.0 0.647 G -66.0 -22.6 NA NA NA
ASP F:140 D:140 104.0 0.693 G -69.6 -20.1 NA NA NA
5m2i 1 P01375 100.0 1e-114 X-RAY
 2017-08-30 ASP A:140 A:140 96.0 0.64 G -66.9 -18.0 55.0 0.367 0.273 H:None:0.273
 HOH
 3.027
 OD2
 O
ASP B:140 B:140 95.0 0.633 G -67.8 -16.4 59.0 0.393 0.24 G:None:0.24
 HOH
 3.111
 O
 O
ASP C:140 C:140 94.0 0.627 G -68.9 -16.2 59.0 0.393 0.234 I:None:0.233
 HOH
 3.437
 OD2
 O
ASP D:140 D:140 98.0 0.653 G -66.8 -17.9 NA NA NA
ASP E:140 E:140 92.0 0.613 G -66.6 -17.7 NA NA NA
ASP F:140 F:140 95.0 0.633 G -68.2 -16.7 NA NA NA
5m2j 1 P01375 100.0 1e-114 X-RAY
 2017-08-30 ASP A:140 A:140 100.0 0.667 G -67.9 -18.4 100.0 0.667 0.0 HOH
 2.726
 O
 O
5m2m 1 P01375 100.0 1e-114 X-RAY
 2017-08-30 ASP A:140 A:140 108.0 0.72 G -66.5 -20.2 0.0 0.0 0.72 F:None:0.72
 HOH
 4.662
 N
 O
ASP B:140 B:140 110.0 0.733 G -67.0 -19.3 0.0 0.0 0.733 D:None:0.733
 HOH
 3.123
 O
 O
ASP C:140 C:140 108.0 0.72 G -67.4 -20.4 0.0 0.0 0.72 E:None:0.72
 HOH
 3.326
 O
 O
ASP G:140 G:140 109.0 0.727 G -66.4 -20.4 NA NA NA
ASP I:140 I:140 109.0 0.727 G -66.1 -21.0 NA NA NA
ASP K:140 M:140 128.0 0.853 -69.1 360.0 NA NA NA
5wux 3 C1K3N5 100.0 1e-114 X-RAY
 2017-06-07 ASP G:140 E:140 83.0 0.553 G -80.4 -5.2 NA NA NA
ASP H:140 F:140 95.0 0.633 G -65.8 -28.8 50.0 0.333 0.3 B:None:0.3
ASP I:140 G:140 93.0 0.62 G -51.2 -39.6 NA NA NA
6ooy 1 P01375 100.0 1e-114 X-RAY
 2019-12-25 ASP A:140 A:140 104.0 0.693 T -72.1 -22.7 104.0 0.693 0.0 HOH
 8.248
 N
 O
ASP B:140 B:140 105.0 0.7 G -60.2 -21.3 105.0 0.7 0.0 HOH
 4.266
 O
 O
ASP C:140 C:140 97.0 0.647 T -76.2 -15.4 97.0 0.647 0.0
6ooz 1 P01375 100.0 1e-114 X-RAY
 2019-12-25 ASP A:140 A:140 103.0 0.687 G -60.4 -21.2 103.0 0.687 0.0 HOH
 9.235
 N
 O
ASP B:140 B:140 106.0 0.707 G -52.5 -29.3 106.0 0.707 0.0 HOH
 3.821
 O
 O
ASP C:140 C:140 105.0 0.7 G -54.7 -30.4 105.0 0.7 0.0 HOH
 2.877
 OD2
 O
6rmj 1 P01375 99.37 3e-114 X-RAY
 2019-10-09 ASP A:146 A:140 90.0 0.6 G -68.7 -18.2 90.0 0.6 0.0 HOH
 6.034
 O
 O
ASP B:146 B:140 98.0 0.653 G -70.0 -17.8 98.0 0.653 0.0 HOH
 7.569
 N
 O
ASP C:146 C:140 102.0 0.68 G -69.9 -16.8 102.0 0.68 0.0 HOH
 8.810
 H
 O
5uui 1 P01375 98.73 1e-113 X-RAY
 2017-08-02 ASP A:141 A:140 97.0 0.647 G -73.0 -9.8 97.0 0.647 0.0 HOH
 2.572
 OD1
 O
1a8m 1 P01375 99.36 1e-113 X-RAY
 1998-06-17 ASP A:140 A:140 103.0 0.687 G -63.2 -15.9 103.0 0.687 0.0 HOH
 8.896
 N
 O
ASP B:140 B:140 88.0 0.587 T -77.7 -34.7 88.0 0.587 0.0 HOH
 4.160
 CB
 O
ASP C:140 C:140 101.0 0.673 T -87.5 -7.5 101.0 0.673 0.0 HOH
 3.654
 CB
 O
3wd5 1 P01375 99.36 1e-113 X-RAY
 2013-08-14 ASP A:140 A:140 96.0 0.64 G -80.2 -3.7 68.0 0.453 0.187 B:None:0.187
 HOH
 7.334
 O
 O
1tnf 1 P01375 99.36 5e-113 X-RAY
 1990-01-15 ASP A:140 A:140 105.0 0.7 G -55.4 -26.1 105.0 0.7 0.0
ASP B:140 B:140 95.0 0.633 G -62.7 -13.1 95.0 0.633 0.0
ASP C:140 C:140 100.0 0.667 G -69.3 -36.4 100.0 0.667 0.0
2tun 1 P01375 98.73 2e-112 X-RAY
 1994-01-31 ASP A:140 A:140 92.0 0.613 G -63.7 -30.0 58.0 0.387 0.226 D:P01375:0.227
ASP B:140 B:140 101.0 0.673 T -96.0 -34.4 101.0 0.673 0.0
ASP C:140 C:140 90.0 0.6 T -71.7 32.4 90.0 0.6 0.0
ASP D:140 D:140 88.0 0.587 G -77.7 16.0 54.0 0.36 0.227 A:P01375:0.227
ASP E:140 E:140 74.0 0.493 T -72.6 -49.5 74.0 0.493 0.0
ASP F:140 F:140 92.0 0.613 G -59.7 1.0 92.0 0.613 0.0
3it8 1 P01375 99.34 1e-109 X-RAY
 2009-10-20 ASP A:144 A:140 94.0 0.627 G -76.4 -10.5 94.0 0.627 0.0 HOH
 8.701
 N
 O
ASP B:144 B:140 93.0 0.62 G -75.6 -11.9 93.0 0.62 0.0 HOH
 8.833
 N
 O
ASP C:144 C:140 95.0 0.633 G -76.3 -11.0 95.0 0.633 0.0 HOH
 8.568
 N
 O
ASP G:144 G:140 94.0 0.627 G -76.3 -10.4 NA NA NA
ASP H:144 H:140 95.0 0.633 G -76.7 -10.7 NA NA NA
ASP I:144 I:140 94.0 0.627 G -76.0 -10.9 NA NA NA
3alq 1 P01375 96.18 2e-109 X-RAY
 2010-11-17 ASP A:140 A:140 98.0 0.653 G -76.9 -2.8 98.0 0.653 0.0
ASP B:140 B:140 99.0 0.66 G -69.8 -3.6 99.0 0.66 0.0
ASP C:140 C:140 96.0 0.64 G -67.8 -10.0 96.0 0.64 0.0 HOH
 9.091
 O
 O
ASP D:140 D:140 95.0 0.633 G -68.4 -13.5 NA NA NA
ASP E:140 E:140 93.0 0.62 G -81.6 -7.4 NA NA NA
ASP F:140 F:140 95.0 0.633 T -82.5 -3.2 NA NA NA
3l9j 2 P01375 100.0 1e-108 X-RAY
 2010-02-23 ASP B:132 T:140 99.0 0.66 G -70.2 -20.1 11.0 0.073 0.587 A:None:0.587
 HOH
 3.311
 OD1
 O
2az5 1 P01375 100.0 6e-108 X-RAY
 2005-11-29 ASP A:131 A:140 102.0 0.68 G -64.4 -20.7 102.0 0.68 0.0 HOH
 2.460
 OD1
 O
ASP B:131 B:140 104.0 0.693 G -70.2 -22.7 104.0 0.693 0.0 HOH
 2.199
 OD1
 O
ASP C:131 C:140 101.0 0.673 G -62.1 -27.3 101.0 0.673 0.0 HOH
 6.799
 N
 O
ASP D:131 D:140 103.0 0.687 G -71.8 -19.7 103.0 0.687 0.0 HOH
 3.688
 O
 O
4tsv 1 P01375 98.0 6e-107 X-RAY
 1998-12-30 ASP A:133 A:140 97.0 0.647 G -78.9 -5.2 97.0 0.647 0.0 HOH
 3.094
 O
 O
5tsw 1 P01375 98.0 6e-107 X-RAY
 1999-05-07 ASP A:133 A:140 99.0 0.66 T -78.1 -7.7 99.0 0.66 0.0 HOH
 6.552
 OD1
 H2
ASP B:133 B:140 93.0 0.62 G -66.9 -24.8 93.0 0.62 0.0 HOH
 2.859
 O
 H1
ASP C:133 C:140 93.0 0.62 T -78.5 -15.3 93.0 0.62 0.0 HOH
 2.779
 CB
 H1
ASP D:133 D:140 100.0 0.667 T -82.2 -9.0 NA NA NA
ASP E:133 E:140 90.0 0.6 G -65.1 -36.3 NA NA NA
ASP F:133 F:140 95.0 0.633 G -70.1 -16.8 NA NA NA
2e7a 1 P01375 92.36 9e-106 X-RAY
 2007-11-13 ASP A:140 A:140 98.0 0.653 G -71.3 -11.0 98.0 0.653 0.0 HOH
 2.627
 OD2
 O
ASP B:140 B:140 92.0 0.613 G -68.0 -20.9 92.0 0.613 0.0 HOH
 2.868
 OD2
 O
ASP C:140 C:140 95.0 0.633 T -73.1 -9.5 95.0 0.633 0.0 HOH
 3.516
 C
 O
2zjc 1 P01375 92.99 3e-105 X-RAY
 2009-01-20 ASP A:140 A:140 99.0 0.66 G -75.4 7.4 99.0 0.66 0.0 HOH
 6.914
 OD1
 O
ASP B:140 B:140 70.0 0.467 T -76.3 -19.7 70.0 0.467 0.0 HOH
 2.602
 OD2
 O
ASP C:140 C:140 94.0 0.627 G -74.9 5.7 94.0 0.627 0.0 HOH
 4.497
 O
 O
2zpx 1 P01375 92.36 3e-104 X-RAY
 2009-03-24 ASP A:140 A:140 98.0 0.653 T -93.1 -22.1 98.0 0.653 0.0 HOH
 4.172
 O
 O
ASP B:140 B:140 107.0 0.713 T -94.3 -22.1 107.0 0.713 0.0
ASP C:140 C:140 103.0 0.687 T -108.9 3.5 103.0 0.687 0.0
7kp7 1 P06804 80.54 4e-88 X-RAY
 2021-01-13 LYS A:131 B:140 148.0 0.74 G -55.8 -28.9 148.0 0.74 0.0 HOH
 3.028
 N
 O
LYS B:131 A:140 80.0 NA G -54.2 -29.9 80.0 NA NA HOH
 3.167
 N
 O
LYS C:131 C:140 80.0 NA G -54.1 -30.9 80.0 NA NA HOH
 2.881
 N
 O
7kp8 1 P06804 80.41 1e-87 X-RAY
 2021-01-13 LYS A:130 A:139 88.0 NA G -55.2 -31.1 88.0 NA NA
LYS B:130 B:139 84.0 NA G -55.4 -30.0 84.0 NA NA
LYS C:130 C:139 86.0 NA G -54.2 -30.9 86.0 NA NA

AlphaFold DB

Entity Residue Monomer View
ID Entity Uniprot Identity Evalue Name Label Auth ASA rASA SS φ ψ
af-p01375-f1 1 P01375 100.0 2e-174 ASP A:216 A:216 106.0 0.707 G -61.1 -21.1